===== Variations and Add-ons =====

The diSPIM is inherently modular and easy to modify.  Here are some variations on the basic diSPIM system already being used:
  * single-sided system with high-NA imaging objective (e.g. same objective used for lattice light sheet)
  * moving sample through fixed light sheet with XY stage
      * useful especially for flat samples so beam waist can stay on coverslip and sample size is not limited by piezo travel
  * emission filter wheels for better channel isolation
  * turret on bottom for different objectives (useful especially when doing photomanipulation)
  * photomanipulation from inverted microscope objective using ASI scanner and [[https://micro-manager.org/wiki/Projector_Plugin|Projector plugin]] in Micro-Manager 
      * possible using other home-built or commercial scanned illumination products as well
  * gated laser illumination in certain range of slices to avoid stimulating photosensitive section of sample
  * "anti-striping" or altering the angle of the beam to avoid shadowing effects

Other variations are in development by ASI, Hari Shroff's lab, and others.  What do you want to do?