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docs:comparison_with_confocal [2015/06/18 22:23] Jon Daniels added Figure 3 to SDCM bleaching |
docs:comparison_with_confocal [2016/01/26 17:51] Jon Daniels |
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===== Comparison of diSPIM With Confocal ===== | ===== Comparison of diSPIM With Confocal ===== | ||
- | The following comparison was written with diSPIM in mind, but the main points apply to all types of SPIM. | + | The following comparison was written with diSPIM in mind, but the main points apply to all types of SPIM/LSFM. |
Spinning disk confocal (SDCM) is just a massively parallel confocal implementation so it's faster but otherwise has the some characteristics as laser scanning confocal. | Spinning disk confocal (SDCM) is just a massively parallel confocal implementation so it's faster but otherwise has the some characteristics as laser scanning confocal. | ||
- | * **Light dose: | + | * **Light dose: |
* A rule of thumb is that you need X times as much light for confocal as you do for SPIM where X is the number of slices in your stack. | * A rule of thumb is that you need X times as much light for confocal as you do for SPIM where X is the number of slices in your stack. | ||
- | * [[http:// | + | * [[http:// |
- | * **Z resolution: | + | * **Z resolution: |
* Using Bessel beams to create the sheet can improve Z-resolution for single-view SPIM, but won't meaningfully change dual view resolution after registration/ | * Using Bessel beams to create the sheet can improve Z-resolution for single-view SPIM, but won't meaningfully change dual view resolution after registration/ | ||
* **XY resolution: | * **XY resolution: | ||
- | * **Speed:** SPIM (per view) and SDCM have comparable speed (assuming | + | * **Speed:** SPIM (per view) and SDCM have comparable speed if SDCM laser intensity |
* Camera readout speed bounds the maximum achievable frame rate. For example, 512 pixels high ROI is 2.5 ms readout time for sCMOS. | * Camera readout speed bounds the maximum achievable frame rate. For example, 512 pixels high ROI is 2.5 ms readout time for sCMOS. |