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docs:comparison_with_confocal [2015/08/06 20:04]
Jon Daniels 		docs:comparison_with_confocal [2015/08/25 16:45]
Jon Daniels
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 Spinning disk confocal (SDCM) is just a massively parallel confocal implementation so it's faster but otherwise has the some characteristics as laser scanning confocal. Spinning disk confocal (SDCM) is just a massively parallel confocal implementation so it's faster but otherwise has the some characteristics as laser scanning confocal.
  
-  * **Light dose:** Confocal has much more photobleaching/phototoxicity than SPIM (i.e. SPIM uses the excitation light much more efficiently).  Low light dose is the main motivation for most SPIM users.+  * **Light dose:** diSPIM has much lower dose and hence less photobleaching/phototoxicity than confocal. Reducing light dose is the main motivation for most SPIM users.
       * A rule of thumb is that you need X times as much light for confocal as you do for SPIM where X is the number of slices in your stack.       * A rule of thumb is that you need X times as much light for confocal as you do for SPIM where X is the number of slices in your stack.
       * [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_F3.html|Figure 3]] and [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_SV2.html|Supplementary Video 2]] of Wu et al. offer bleaching comparisons of imaging with diSPIM and SDCM.  Other papers such as [[http://dx.doi.org/10.1002/jbio.201200144|this one]] offer comparisons of light sheet with confocal which should apply to diSPIM.       * [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_F3.html|Figure 3]] and [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_SV2.html|Supplementary Video 2]] of Wu et al. offer bleaching comparisons of imaging with diSPIM and SDCM.  Other papers such as [[http://dx.doi.org/10.1002/jbio.201200144|this one]] offer comparisons of light sheet with confocal which should apply to diSPIM.

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