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docs:mm_dispim_plugin_user_guide [2019/05/30 09:12]
Jon Daniels [Acquisition Mode] |
docs:mm_dispim_plugin_user_guide [2019/07/03 00:03]
Jon Daniels [Acquisition Mode] |
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| Currently several modes are supported: | Currently several modes are supported: |
| * Synchronous piezo/slice scan: standard use, the piezo and sheet move together through the sample | * Synchronous piezo/slice scan: standard piezo use, the piezo and sheet move together through the sample |
| * No scan (fixed sheet): neither the piezo nor light sheet moves; useful for characterizing vibration in the system | * No scan (fixed sheet): none of the piezo nor light sheet nor the stage moves (only the light sheet generation using the sheet axis); useful for characterizing vibration in the system |
| * Stage scan: uses the XY stage to move the sample through a fixed light sheet; for two-sided acquisitions, one view is collected in the first pass, and the second view is collected as the stage moves “back” for a second pass; see the document on stage scanning for details and requirements | * Stage scan: uses the XY stage to move the sample through a fixed light sheet; for two-sided acquisitions, one view is collected in the first pass, and the second view is collected as the stage moves “back” for a second pass; see the document on stage scanning for details and requirements |
| * Stage scan interleaved: same as stage scan except that the stage moves half as fast and the two light paths alternate, meaning both views are collected in the same pass; use caution if you are using a galvo to switch the laser between the sides, this can overstress some galvos | * Stage scan interleaved: same as stage scan except that the stage moves half as fast and the two light paths alternate, meaning both views are collected in the same pass; use caution if you are using a galvo to switch the laser between the sides, this can overstress some galvos |
| * Stage scan unidirectional: same as stage scan except the second view is taken while scanning in the same direction as the first view with a rewind step between. For 1-sided acquisitions stage scan and stage scan unidirectional are equivalent. For 2-sided acquisitions the stage scan unidirectional will take slightly longer (the rewind step is done at 75% of the max stage speed) but makes data processing easier. | * Stage scan unidirectional: same as stage scan except the second view is taken while scanning in the same direction as the first view with a rewind step between. For 1-sided acquisitions stage scan and stage scan unidirectional are equivalent. For 2-sided acquisitions the stage scan unidirectional will take slightly longer (the rewind step is done at 75% of the max stage speed) but makes data processing easier. |
| * Slice scan only: suppresses movement of piezo but moves the light sheet; useful for characterizing light sheet thickness | * Slice scan only: suppresses movement of piezo but moves the light sheet; useful for characterizing light sheet thickness and for autofocus |
| * Piezo scan only: the light sheet stays stationary but the piezo moves; useful for establishing axial PSF | * Piezo scan only: the light sheet stays stationary but the piezo moves; useful for establishing axial PSF and for autofocus |
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