http://dispim.org/ Thu, 30 Apr 2026 04:07:58 +0000 FeedCreator 1.8 http://dispim.org/ttp://dispim.org/lib/tpl/monobook/images/favicon.ico http://dispim.org/ http://dispim.org/docs/comparison_light_sheet_methods?rev=1500581490 Comparison of Light Sheet Microscopy Methods Commercial Light Sheet Microscopes Type # Views Mounting Software Comments anonymous@undisclosed.example.com (Anonymous) docs Thu, 20 Jul 2017 20:11:30 +0000 http://dispim.org/docs/comparison_with_confocal?rev=1462835885 Comparison of diSPIM With Confocal The following comparison was written with diSPIM in mind, but the main points apply to all types of SPIM/LSFM. Spinning disk confocal (SDCM) is just a massively parallel confocal implementation so it's faster but otherwise has the same characteristics as laser scanning confocal. anonymous@undisclosed.example.com (Anonymous) docs Mon, 09 May 2016 23:18:05 +0000 http://dispim.org/docs/deskew?rev=1764716683 Deskewing This page is an overview of deskewing. Image viewers such as FIJI assume that stacks of camera images are formed using motion orthogonal to the image plane, but that assumption is not true for single-objective light sheet, lattice light sheet, and diSPIM with stage scanning. anonymous@undisclosed.example.com (Anonymous) docs Tue, 02 Dec 2025 23:04:43 +0000 http://dispim.org/docs/gaussian_beam?rev=1557271124 Gaussian Beam In most cases the diSPIM has been used with Gaussian beams. As the beam waist gets thinner, the distance over which the beam/sheet is thin (called the confocal length) gets smaller according to well-known equations. Thus there is a fundamental trade-off between the optical sectioning and the field of view. This tradeoff is controlled by the illumination beam's numerical aperture; in the ASI scanner there is an iris and/or aperture to adjust this. For a sheet created by a swept… anonymous@undisclosed.example.com (Anonymous) docs Tue, 07 May 2019 23:18:44 +0000 http://dispim.org/docs/manual?rev=1552322224 diSPIM Manual The diSPIM is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (SPIM) that allows for dual views (d) of a sample mounted on an inverted (i) microscope. This document concentrates on the assembly, setup, and alignment of the diSPIM. The vast majority of these instructions apply regardless of the inverted microscope frame on which the SPIM head is mounted, but configuration specific manuals are in the works. anonymous@undisclosed.example.com (Anonymous) docs Mon, 11 Mar 2019 16:37:04 +0000 http://dispim.org/docs/mm_dispim_plugin_user_guide?rev=1747761528 Micro-Manager diSPIM Plugin User Guide The diSPIM plugin adds functionality to Micro-Manager from alignment to acquisition. This user guide gives an introduction to the utility of each of the tabbed panels in the plugin, as well as guidance on hardware- and sample-based considerations. anonymous@undisclosed.example.com (Anonymous) docs Tue, 20 May 2025 17:18:48 +0000 http://dispim.org/docs/ospim?rev=1667346288 oSpim oSPIM stands for Oblique SPIM. The light sheet is generated using an objective below the sample (an oil objective) and is tilted partway towards TIRF not unlike HiLo illumination. The imaging objective is a water dipping objective that is lowered into the media orthogonal to the light sheet. The oSPIM is an ideal tool for cell biologists or other applications where the sample is in close contact with the coverslip. The same concept was independently and concurrently developed by a res… anonymous@undisclosed.example.com (Anonymous) docs Tue, 01 Nov 2022 23:44:48 +0000 http://dispim.org/docs/sample_prep?rev=1463679207 Sample Prep The page has been created, you can go ahead and edit it. Start by simply entering your text in this edit box. The icons above the edit box will help with the most common markup tasks (bold, lists, links, etc.), plus there is a wiki syntax page with more details about markup. anonymous@undisclosed.example.com (Anonymous) docs Thu, 19 May 2016 17:33:27 +0000 http://dispim.org/docs/start?rev=1435256131 Docs docs index anonymous@undisclosed.example.com (Anonymous) docs Thu, 25 Jun 2015 18:15:31 +0000 http://dispim.org/docs/system_integrators?rev=1507537666 System Integrators diSPIM systems can be purchased from the following companies: * Applied Scientific Instrumentation (ASI) * originator of microscope hardware regardless of the integrator * offers integration services, but no on-site support * demos available in Eugene Oregon USA anonymous@undisclosed.example.com (Anonymous) docs Mon, 09 Oct 2017 08:27:46 +0000 http://dispim.org/docs/user_list?rev=1505752523 diSPIM User List Here is a list of labs with working diSPIM systems. If your lab is missing please add it! * University of Heidelberg, Intelligent Imaging Group (PI Christian Conrad) * NIH, lab of Hari Shroff (4+ experimental systems) * University of Washington, Waterston Lab anonymous@undisclosed.example.com (Anonymous) docs Mon, 18 Sep 2017 16:35:23 +0000