diSPIM Wiki docs:manual

docs:manual:alignment

anonymous@undisclosed.example.com (Anonymous) — Wed, 19 Aug 2020 02:50:14 +0000

diSPIM Alignment (deprecated) docs:manual#optical_alignment Overview Before acquiring data the optical paths of the diSPIM must be aligned. This is the trickiest thing for new diSPIM users to learn. If the system is not disturbed then the alignment can last for months. Changing objectives, crashes, filter changes, and so forth will usually require at least partial realignment.

docs:manual:assembly

anonymous@undisclosed.example.com (Anonymous) — Fri, 26 Jun 2015 20:17:11 +0000

Assembly The steps in this section are demonstrated in the following YouTube video: . Attach the SPIM assembly to the microscope frame The SPIM assembly is shipped separately and mostly assembled. The RAMM frame stand or the microscope mount assembly has two vertical arms on the back where 2 dowel pins each (total of 4) connect the microscope frame to the SPIM-MOUNT . Slide the complete SPIM assembly onto the dowel pins and fastened in place with t…

docs:manual:mmplugin

anonymous@undisclosed.example.com (Anonymous) — Thu, 25 Jun 2015 23:26:46 +0000

Micro-manager diSPIM Plugin Micro-Manager comes with a plugin providing a GUI which facilitates alignment and use of the diSPIM system. Micro-Manager is a free and open-source software package with many capabilities available at . If you are not familiar with Micro-Manager it is worth reading the

docs:manual:overview

anonymous@undisclosed.example.com (Anonymous) — Thu, 25 Jun 2015 20:16:01 +0000

Overview The diSPIM is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (SPIM) that allows for dual views (d) of the sample while mounted on an inverted (i) microscope. The diSPIM has two (usually symmetric) optical paths for light sheet imaging. Two objectives are placed at right angles above a sample mounted horizontally. A light sheet is created from one objective and then imaged using the other objective. A stack of images is collected by moving the ligh…

docs:manual:requirements

anonymous@undisclosed.example.com (Anonymous) — Wed, 14 Dec 2022 21:04:29 +0000

Requirements These are suggestions, for people who want numbers... Room The microscope should be installed in a room with a closable door that is climate controlled. Room lights should be turned off during microscope operation. There should be 0.5 m of space on all sides of the air table.

docs:manual:stage_limits

anonymous@undisclosed.example.com (Anonymous) — Thu, 25 Jun 2015 23:41:48 +0000

Set the Stage Limits When first setting up your microscope you should set the limit magnets on the motorized stages to prevent crashes that can break coverslips or worse. This is especially important when using the 24 x 50 mm coverslip chamber, where there is not much lateral travel room and the coverslip glass is easily broken. Although the TG-1000 firmware supports software-defined limits, setting hardware limits using the provided magnets is the most foolproof way to prevent crashes.