http://dispim.org/ Thu, 09 Apr 2026 09:53:45 +0000 FeedCreator 1.8 http://dispim.org/ttp://dispim.org/lib/tpl/monobook/images/favicon.ico http://dispim.org/ http://dispim.org/hardware/computer?rev=1707933312 Computer Requirements Acquisition Make sure to get a computer with sufficient PCI/PCIe slots for the camera framegrabber cards (usually 2 cameras/cards for dual-view) plus whatever other peripherals you need. Otherwise the main requirement having sufficiently fast disk write speed to handle the camera data. Depending on the use case, solid state drives (SSDs) and/or RAID0 with SSDs may or may not be required. Individual users should consider their requirements. anonymous@undisclosed.example.com (Anonymous) hardware Wed, 14 Feb 2024 17:55:12 +0000 http://dispim.org/hardware/controller?rev=1742580843 Controller The diSPIM uses ASI's modular controller, the TG-1000 or “Tiger” controller. It has separate cards for controlling the XY stage, linear stages, micro-mirrors (scanners), triggering cameras/lasers, driving piezo objective movers if present, driving filterwheels if present, etc. anonymous@undisclosed.example.com (Anonymous) hardware Fri, 21 Mar 2025 18:14:03 +0000 http://dispim.org/hardware/dated?rev=1547840555 Dated Hardware Piezos The method for inserting the objectives was significantly improved in the piezo objective mover (POM) mounting scheme introduced in early 2015. The main benefit is easier objective alignment due to the replacement of the AOA objective adjuster with a smooth linear stage, but other notable benefits include: anonymous@undisclosed.example.com (Anonymous) hardware Fri, 18 Jan 2019 19:42:35 +0000 http://dispim.org/hardware/environmental_control?rev=1605773031 Environmental Control For long-term imaging of some samples the environment must be tightly controlled, including temperature, humidity, and gas concentrations (e.g. CO2). Temperature can either be controlled locally around the sample or the bulk of the microscope can be enclosed and kept at a constant temperature. ASI makes an incubator enclosure that fits around the entire diSPIM on RAMM. Incubator boxes for diSPIM on other inverted microscopes are available from by 3rd parties. anonymous@undisclosed.example.com (Anonymous) hardware Thu, 19 Nov 2020 08:03:51 +0000 http://dispim.org/hardware/lasers?rev=1707778627 Lasers Various laser launches can be used with diSPIM. Below find notes specific to each one. In normal diSPIM use the laser launch is controlled using digital TTL inputs. The Tiger controller's PLC card generates those signals, which will turn the lasers on/off at the appropriate times and also selects the correct side if needed. For information on connecting the cables see the appropriate anonymous@undisclosed.example.com (Anonymous) hardware Mon, 12 Feb 2024 22:57:07 +0000 http://dispim.org/hardware/objectives?rev=1743434586 Objectives The choice of light sheet objectives is limited because they must be co-focused without bumping into each other. See more details below on this page in the section Mechanical angle. The most commonly-used objectives for (symmetric) diSPIM are 40x water-dipping objectives with a NA of 0.8 (Nikon CFI Apo 40XW NIR). Other possibilities include the Olympus 20x/0.5 water (UMPLFLN20XW) anonymous@undisclosed.example.com (Anonymous) hardware Mon, 31 Mar 2025 15:23:06 +0000 http://dispim.org/hardware/piezos?rev=1581625743 Piezos The piezo objective movers are the most fragile part of the system you should exercise caution with them both mechanically and electrically. If you think you have a failure, first follow the troubleshooting instructions below and then contact your system integrator for further help. anonymous@undisclosed.example.com (Anonymous) hardware Thu, 13 Feb 2020 20:29:03 +0000 http://dispim.org/hardware/sample_holder?rev=1567799234 Sample Holder The original sample holder for the diSPIM is an autoclavable stainless steel chamber made by ASI (I-2450). It uses a 24×50 mm coverslip as a transparent bottom. There is only ~5mm travel in both X and Y when 40x/0.8 WD objectives are being used; it was designed to minimize the fluid volume (only a few milliliters). Use the I-3078 stage insert. Contact ASI for CAD files. anonymous@undisclosed.example.com (Anonymous) hardware Fri, 06 Sep 2019 19:47:14 +0000 http://dispim.org/hardware/scanner?rev=1613228761 Scanner The very initial iSPIM and diSPIM in the Shroff lab used a homebuilt scanner system with galvo. ASI developed a compact fiber-coupled “scanner” that uses a tip/tilt MEMS mirror to move a beam to create a light sheet (patent US10168519B2). One axis of the MEMS mirror is moved rapidly to create a virtual light sheet anonymous@undisclosed.example.com (Anonymous) hardware Sat, 13 Feb 2021 15:06:01 +0000 http://dispim.org/hardware/start?rev=1431985771 Hardware hardware index anonymous@undisclosed.example.com (Anonymous) hardware Mon, 18 May 2015 21:49:31 +0000 http://dispim.org/hardware/troubleshooting?rev=1705678200 Troubleshooting The golden rule of troubleshooting is to bisect the issue until the root cause is clear. Below are some hints. In case of communication errors between software and hardware, first power cycle the affected hardware and restart the software. If that doesn't resolve things make sure you can communicate with the specific piece of hardware using manufacturer's software (or for Tiger controller, with a serial terminal). anonymous@undisclosed.example.com (Anonymous) hardware Fri, 19 Jan 2024 15:30:00 +0000 http://dispim.org/hardware/variations?rev=1456956992 Variations and Add-ons The diSPIM is inherently modular and easy to modify. Here are some variations on the basic diSPIM system already being used: * single-sided system with high-NA imaging objective (e.g. same objective used for lattice light sheet) anonymous@undisclosed.example.com (Anonymous) hardware Wed, 02 Mar 2016 22:16:32 +0000