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hardware:computer [2018/10/16 23:32]
Jon Daniels [Specific suggestions] |
hardware:computer [2020/12/05 05:25]
Jon Daniels [Acquisition] |
| The sCMOS cameras used with diSPIM can generate 800 MB/sec (100 fps at 4 MP, 16 bits per pixel). However the maximum possible frame rate of the camera is not achieved for diSPIM.((Light sheet illumination only occurs during global exposure, and camera-limited frame rates occur without any global exposure time.)) Typical maximum acquisition speeds are 1024x1024 at 50fps or 512x512 at 200 fps; both these situations both generate 100MB/sec. The average data rate, and hence hard drive speed requirement, is usually even less because most commonly acquisition occurs in bursts (i.e. there is time between successive time points) and a RAM buffer initially holds images so the hard drive needs to keep up with the average data rate. At present only one camera works at a time, though in the future there are schemes where both cameras could be used simultaneously and thus double the data rate. | The sCMOS cameras used with diSPIM can generate 800 MB/sec (100 fps at 4 MP, 16 bits per pixel). However the maximum possible frame rate of the camera is not achieved for diSPIM.((Light sheet illumination only occurs during global exposure, and camera-limited frame rates occur without any global exposure time.)) Typical maximum acquisition speeds are 1024x1024 at 50fps or 512x512 at 200 fps; both these situations both generate 100MB/sec. The average data rate, and hence hard drive speed requirement, is usually even less because most commonly acquisition occurs in bursts (i.e. there is time between successive time points) and a RAM buffer initially holds images so the hard drive needs to keep up with the average data rate. At present only one camera works at a time, though in the future there are schemes where both cameras could be used simultaneously and thus double the data rate. |
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| 100 MB/sec is typical for a magnetic hard drive. 300 MB/sec is typical for a single SSD. If the data rate is too high for a single SSD, use SSDs in RAID0 configuration (e.g. 4 SSDs in RAID0 can achieve >1 GB/s). To benchmark your PC's hard drive write speed you can use [[http://crystalmark.info/?lang=en | Crystal Disk Mark]]. I'm pretty sure the relevant score to diSPIM acquisition is the "Seq" "Write" score (Sequential (Block Size=1MiB) Read/Write with single Thread), at least for Micro-manager software with typical acquisition settings. | 100 MB/sec is typical for a magnetic hard drive. 300 MB/sec is typical for a single SSD. If the data rate is too high for a single SSD, use SSDs in RAID0 configuration (e.g. 4 SSDs in RAID0 can achieve >1 GB/s). Lately M.2 drives with comparable speeds to a RAID0 with SSDs have become available and might be a good option. To benchmark your PC's hard drive write speed you can use [[http://crystalmark.info/?lang=en | Crystal Disk Mark]]. I'm pretty sure the relevant score to diSPIM acquisition is the "Seq" "Write" score (Sequential (Block Size=1MiB) Read/Write with single Thread), at least for Micro-manager software with typical acquisition settings. |
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