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start [2017/11/15 04:40]
Jon Daniels 		start [2018/02/21 22:59]
Jon Daniels [The Idea]
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 ===== The Idea ===== ===== The Idea =====
  
-The //diSPIM// is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (//SPIM//) that allows for dual views (//d//) of the sample while mounted on an inverted (//i//) microscope.  The diSPIM was co-developed by the lab of [[http://www.nibib.nih.gov/about-nibib/staff/hari-shroff|Hari Shroff]] at NIH/NIBIB and [[http://www.asiimaging.com/|Applied Scientific Instrumentation]] (ASI).  SPIM is also referred to as [[http://en.wikipedia.org/wiki/Light_sheet_fluorescence_microscopy|light sheet fluorescence microscopy]] or LSFM because it uses a sheet or plane of light to illuminate the sample perpendicular to the imaging direction.+The //diSPIM// is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (//SPIM//) that allows for dual views (//d//) of the sample while mounted on an inverted (//i//) microscope (i.e. the SPIM objectives are upright).  The diSPIM was co-developed by the lab of [[http://www.nibib.nih.gov/about-nibib/staff/hari-shroff|Hari Shroff]] at NIH/NIBIB and [[http://www.asiimaging.com/|Applied Scientific Instrumentation]] (ASI).  SPIM is also referred to as [[http://en.wikipedia.org/wiki/Light_sheet_fluorescence_microscopy|light sheet fluorescence microscopy]] or LSFM because it uses a sheet or plane of light to illuminate the sample perpendicular to the imaging direction.
  
 ===== The Implementation ===== ===== The Implementation =====

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