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docs:comparison_light_sheet_methods [2016/05/09 21:10] Jon Daniels [Detailed Comparison] |
docs:comparison_light_sheet_methods [2017/04/26 21:41] Jon Daniels [Detailed Comparison] |
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| LaVision BioTec Ultramicroscope | | LaVision BioTec Ultramicroscope | ||
- | In general the diSPIM approach is ideal for cells or small groups of cells (e.g. c. elegans embryos). | + | In general the diSPIM approach is ideal for cells or small groups of cells (e.g. c. elegans embryos). |
Instruments which rely on a single fixed view lead to relatively poor axial resolution and poor imaging of scattering samples. | Instruments which rely on a single fixed view lead to relatively poor axial resolution and poor imaging of scattering samples. | ||
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* can obtain sub-diffraction images like SIM by combining multiple exposures with shifted lattice; cost is extra time/dose like SIM | * can obtain sub-diffraction images like SIM by combining multiple exposures with shifted lattice; cost is extra time/dose like SIM | ||
* objectives dipped in media at angle from above ([[http:// | * objectives dipped in media at angle from above ([[http:// | ||
- | * 25x 1.1 NA detection objective and custom excitation objective for fixed single-view; | + | * 25x 1.1 NA detection objective and custom excitation objective for fixed single-view; |
- | * starting to be commercialized by [[https:// | + | * commercialized by [[https:// |
Note: if anything here is inaccurate please simply create an account and fix it! | Note: if anything here is inaccurate please simply create an account and fix it! | ||