Sample Holder

The original sample holder for the diSPIM is an autoclavable stainless steel chamber made by ASI (I-2450). It uses a 24×50 mm coverslip as a transparent bottom. There is only ~5mm travel in both X and Y when 40x/0.8 WD objectives are being used; it was designed to minimize the fluid volume (only a few milliliters). Use the I-3078 stage insert. Contact ASI for CAD files.

A similar but slightly larger ASI-made sample holder accommodates larger objectives including the Nikon 25x/1.1 WD paired with Special Optics illumination objective (I-2460). It uses a 24×60 mm coverslip as a bottom. Contact ASI for CAD files. Use the I-4020 stage insert.

A petri dish is easily used as a sample holder and ASI makes a variety of stage inserts for all manner of dishes. Just make sure you get one large enough to accommodate the objectives, e.g. 85mm diameter.

Rusty Nicovich designed a large 3D printed chamber that fits into the stage in place of an insert and accommodates the cleared tissue objectives. It has a glass bottom and volume ~190mL. CAD files and more details at https://github.com/PRNicovich/3D-Printed-Optics-Lab-Parts/tree/master/ASI%20Large%20Volume%20Chamber.

ASI designed a large 3D printed chamber that fits into the stage in place of an insert and accommodates the NA 0.4 cleared tissue objectives. There is space to glue in a Thorlabs SB1 or SB1/M kinematic base so the sample can be mounted to the kinematic top and removed and replaced repeatedly, and ~10mm travel in all dimensions. Depending on the 3D printed material it may be useful to seal it with epoxy or some other leak-proof coating. CAD files are available here. ASI also offers a stainless steel sample holder that fits the NA 0.7 multi-immersion objectives with just a few mm of travel (for live imaging).

A key advantage of the oSPIM system is that it can use 35mm glass-bottom dishes.