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docs:comparison_with_confocal [2015/08/04 16:19]
Jon Daniels 		docs:comparison_with_confocal [2015/08/06 20:04]
Jon Daniels
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       * A rule of thumb is that you need X times as much light for confocal as you do for SPIM where X is the number of slices in your stack.       * A rule of thumb is that you need X times as much light for confocal as you do for SPIM where X is the number of slices in your stack.
       * [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_F3.html|Figure 3]] and [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_SV2.html|Supplementary Video 2]] of Wu et al. offer bleaching comparisons of imaging with diSPIM and SDCM.  Other papers such as [[http://dx.doi.org/10.1002/jbio.201200144|this one]] offer comparisons of light sheet with confocal which should apply to diSPIM.       * [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_F3.html|Figure 3]] and [[http://www.nature.com/nbt/journal/v31/n11/fig_tab/nbt.2713_SV2.html|Supplementary Video 2]] of Wu et al. offer bleaching comparisons of imaging with diSPIM and SDCM.  Other papers such as [[http://dx.doi.org/10.1002/jbio.201200144|this one]] offer comparisons of light sheet with confocal which should apply to diSPIM.
-  * **Z resolution:** diSPIM after registration/fusion has best Z-resolution.  Next best is confocal, which in turn has better Z resolution than single-view SPIM.+  * **Z resolution:** diSPIM after registration/fusion has best Z-resolution (same as XY resolution).  Next best is confocal, which in turn has better Z resolution than single-view SPIM.
       * Using Bessel beams to create the sheet can improve Z-resolution for single-view SPIM, but won't meaningfully change dual view resolution after registration/fusion.       * Using Bessel beams to create the sheet can improve Z-resolution for single-view SPIM, but won't meaningfully change dual view resolution after registration/fusion.
   * **XY resolution:** confocal and SPIM are comparable.   * **XY resolution:** confocal and SPIM are comparable.
   * **Speed:** SPIM (per view) and SDCM have comparable speed (assuming SDCM has higher laser intensity to make up for the ~3% open area of the spinning disks).   * **Speed:** SPIM (per view) and SDCM have comparable speed (assuming SDCM has higher laser intensity to make up for the ~3% open area of the spinning disks).
-      * Camera readout speed bounds the maximum achievable frame rate.  For example, 512 pixels high ROI is 2.5 ms readout time for sCMOS.  Allowing 2.5 ms illumination time results in 5 ms total per image or 200 frames per second.+      * Camera readout bounds the maximum achievable frame rate.  For example, 512 pixels high ROI is 2.5 ms readout time for sCMOS.  Allowing 2.5 ms illumination time results in 5 ms total per image or 200 frames per second.

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