Differences

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--- docs:ct-dspim [2019/02/25 18:33]
Melissa Glidewell still in creation mode
+++ docs:ct-dspim [2019/02/28 18:53] (current)
Melissa Glidewell [SPIM Head] adding Post Mounts section
@@ Line 10: / Line 10: @@
 {{section>modular_manual#overview&noheader}}
-<imgcaption SPIM_Figure|Typical complete diSPIM system on RAMM frame showing many major components in common with the CT-dSPIM: 3) SPIM arm mount (RAO-0046), 4) objective mounting assembly, 8) MIM-CUBE-II w/ mirror, 9) MIM-CUBE-II w/ fluorescence filters, 10) C60-TUBE_B imaging tube lens, 11) C60-TUBE_160 scanner tube lens, 12) 50 mm extension tube, 13) MM-SCAN_1M light sheet scanner, 14) MS-2500 XY stage.>{{:docs:manual:dispim_figure.jpg?600| Typical complete diSPIM system on RAMM frame showing major components.}}</imgcaption>
+<imgcaption SPIM_Figure|Typical diSPIM system on RAMM frame showing many major components in common with the CT-dSPIM: 3) SPIM arm mount (RAO-0046), 4) objective mounting assembly, 8) MIM-CUBE-II w/ mirror, 9) MIM-CUBE-II w/ fluorescence filters, 10) C60-TUBE_B imaging tube lens, 11) C60-TUBE_160 scanner tube lens, 12) 50 mm extension tube, 13) MM-SCAN_1M light sheet scanner, 14) MS-2500 XY stage.>{{:docs:manual:dispim_figure.jpg?600| Typical complete diSPIM system on RAMM frame showing major components.}}</imgcaption>
-<imgcaption ctdSPIM|Typical complete ct-dSPIM system with SPIM head supported by stainless steel posts over an XY stage mounted to Z-axis stilts>{{:docs:ct-dspim.jpg?400|Typical ct-dSPIM}}</imgcaption>
+<imgcaption ctdSPIM|Typical ct-dSPIM system has the SPIM head supported by stainless steel posts over a FTP (an XY stage mounted to Z-axis stilts).>{{:docs:ct-dspim.jpg?400|Typical ct-dSPIM}}</imgcaption>
 ====== Assembly ======
@@ Line 18: / Line 18: @@
 ===== FTP (XYZ Stage) =====
-Individually, the stilts of the FTP are linear, or single axis, stages. In the firmware, one of them is designated the master axis and the other the slave.((Usually it is master-Z-axis and slave-F-axis, but it can vary depending on the system.)) Any time [they are both plugged in and] the master is commanded to move, the slave will move as if given the same command.((The only reason to command the slave axis directly is if the stilts' slides are not similarly positioned relative to their respective bodies.))
+**Tools**: 4 mm and 5 mm hex drivers\\
+Individually, the stilts of the FTP are linear, or single axis, stages. In the firmware, one of them is designated the master axis and the other the slave.((Usually it is master-Z-axis and slave-F-axis, but it can vary depending on the system.)) Any time [they are both plugged in and] the master is commanded to move, the slave will move as if given the same command.((The only reason to command the slave axis directly is for alignment purposes.))
 Together, the stilts of the FTP produce movement in the Z-axis, raising and lowering the entire stage, insert/chamber, and sample.
-The XY stage takes care of the rest.
+Mount the stilts to the breadboard, align them if necessary, and mount the XY stage to the stilts. ASI has a detailed [[http://www.asiimaging.com/docs/ftp_setup|FTP setup guide]] on their documentation site.
-ASI has a detailed [[http://www.asiimaging.com/docs/ftp_setup|FTP setup guide]] on their documentation site. It will walk you through mounting the stilts to the breadboard, aligning the stilts if necessary, and mounting the XY stage on top.
+===== Post Mounts =====
+ASI has a detailed [[http://asiimaging.com/docs/post_mount_setup|Post Mount Setup guide]] on their documentation site.
+Mount the post mount rods, ~55 cm center to center, to the optic table with four M6 x 25 mm bolts.
+The retaining collars are already installed on your posts at the same height; they can be adjusted later.
+Place the black rod-to-rod 90° connector on top of the retaining collars on both posts as in Figure 1.  Do not tighten them yet.
+Insert the shorter horizontal rod (attached to a black S-shaped arm with a split ring on the end) all the way into the black connector.
+Tighten the clamp so that the split rings are symmetrical: up ~45°, towards the center, and almost exactly 20 cm center to center (the distance between CUBE-IIIs, center to center).
+Leave the connection around the vertical post loose.
 ===== SPIM Head =====
-{{section>modular_manual#mount_the_spim_head&noheader}}
+**Tools**: 6 hex driver
-\\
+from ct-spim document in Google Drive
-\\
+Post Mount Rods
-\\
-\\
+SPIM Head
-\\
+Attach the camera and scanner tube lenses using a 1.5 mm hex driver, see Figure 3.
-\\
-<imgcaption SPIM_mount_to_RAMM| SPIM head mounts via 2 dowel pins and a bolt at the indicated location regardless of the microscope frame. (RAMM frame pictured)>{{:docs:manual/SPIM_mount_to_RAMM.png| The SPIM head mounted to the microscope frame via 2 dowel pins and a bolt.}}</imgcaption>
+Figure 2: The camera and scanner tube lenses mount via three set screws in the CUBE-IIIs.
+Lift the SPIM head and insert the tube lenses all the way, until the cubes are up against the split rings.  You may need to loosen the clamps around the horizontal bars. Make sure you support the SPIM head any time the horizontal bars are not tightly clamped.
+Level the SPIM head; support its weight and loosen all 4 clamps. After it is level, engage the clamps on all 4 rod connection points. The two sides should be symmetric.
+Tighten the bolts on the split ring, alternately tightening one bolt a bit (less one turn) and then the other, otherwise the asymmetric tension can cause the split ring to bind up.
+If you need to move the SPIM head up or down, support the SPIM head, loosen the retaining collars on the vertical rods and move them along the posts, and finally re-lock onto the vertical rods.
-===== Red Shipping Bracket =====
-{{section>modular_manual#red_bracket&noheader}}
 ===== Scanners =====
 {{section>modular_manual#scanners&noheader}}
-<imgcaption Scanner_assembly|Scanner attached to C-mount adapter; tube lens ready to be installed on the CUBE-III>{{:docs:manual/Scanner_assembly.jpg?700|Scanner attached to C-mount adapter with tube lens assembly ready to be installed.}} </imgcaption>
+<imgcaption Scanner_assembly|Scanner attached to C-mount adapter (left); 160 mm focal length tube lens and 10 mm spacer (right) ready to be mounted>{{:docs:manual/Scanner_assembly.jpg?700|Scanner attached to C-mount adapter (left); tube lens and spacer (right)}} </imgcaption>
-<imgcaption Scanner_tube_install|Scanner tube lens assembly mounted before the scannner with C-mount adapter is installed>{{:docs:manual/Scanner_tube_install.jpg?300|Tube lens assembly is attached to lower cubes on the microscope.}}</imgcaption>
+<imgcaption Scanner_tube_install|160 mm F.L. tube lenses and spacers mount to the lower, or inner, CUBE-IIIs before the scannner / C-mount adapter are installed>{{:docs:manual/Scanner_tube_install.jpg?300|Tube lens assembly attaches to lower cubes on the microscope}}</imgcaption>
 <imgcaption NotWellAligned|Path A scanner with poor alignment>{{:docs:manual/NotWellAligned.jpg?300|Scanner A poorly aligned}}</imgcaption>
 <imgcaption BetterAligned|Path A scanner with good alignment>{{:docs:manual/BetterAligned.jpg?300|Scanner A well aligned}}</imgcaption>
@@ Line 50: / Line 63: @@
 ==== Compatibility ====
 {{section>modular_manual#camera_setup&noheader}}
-==== Mount ====
-Tools: M5 hex driver\\
-In the standard configuration for diSPIM on a RAMM frame, as in <imgref SPIM_Figure>, each camera tube lens is fastened to the SPIM-MOUNT((Other common configurations use a post mount system to minimize vibration from the camera's fans, or to accommodate an FTP system in lieu of a RAMM frame.)) with a ring clamp. The end of the tubes should come within approximately 1 mm from the upper (mirror) CUBE-IIIs. When the objectives are lowered into position for viewing a sample, adjust the bolts holding the tube support arms so the camera tube lenses are centered on the CUBE-IIIs. \\
 ==== Orient & Connect ====
@@ Line 66: / Line 75: @@
 ===== Objectives =====
 {{ youtube>TAgbr6IrTqw?start=889&end=1246&rel=0?small}}
-**Tools**: M1.5 hex driver\\
+**Tools**: 1.5 mm hex driver\\
-The video is cued up to show the process for mounting the objectives with a discussion of the two optical paths, Path A and Path B at the end.\\ \\
+The video is cued up to show the process for mounting the objectives (on a diSPIM) with a discussion of the two optical paths, Path A and Path B at the end.\\ \\
-If your system was delivered before 2015, refer to the document on [[hardware:dated#piezos|pre-2015 piezos]].
-The objective mounting assembly includes a __ob__jective __l__ateral __p__ositioner __a__ssembly (OBLPA) for one side of the SPIM head and a replacement spacer for the other, a piezo objective mover (POM), and the bushing that the objective screws into; see <imgref piezo_with_bushing>. The assembly mounts to the right angle bracket via dovetail joint and is secured into position via set screw.\\
-<imgcaption piezo_with_bushing| Objective mounting assembly top to bottom: objective lateral positioner assembly (OBLPA), piezo objective mover (POM), bushing, objective>{{:docs:manual/piezo_with_bushing.jpg?300| Objective mounting assembly}}</imgcaption>
-Remove either one of the objective mounting assemblies; use a 1.5 mm hex driver to loosen the center set screw on the end of the right angle bracket, as in <imgref piezo_dovetail>. Grip the OBLPA or replacement spacer by the front and back (avoiding unnecessary stress on the piezo), and pull towards you (while pushing on the right angle bracket). Screw an objective into the bushing that is still mounted; add the other objective to the assembly you just removed. Re-insert the assembly (it will “click” into position) and tighten the set screw.
+The objective mounting assembly includes an __ob__jective __l__ateral __p__ositioner __a__ssembly (OBLPA) for one side and a replacement spacer for the other, each with a bushing that the objective screws into. The assembly mounts to the right angle bracket via dovetail joint and is secured into position via set screw. Due to the long working distance of the objectives used by the ct-dSPIM, it is not necessary to remove the objective mounting assemblies in order to install the objectives; if you choose to do so, use a 1.5 mm hex driver to loosen the center set screw on the end of the right angle bracket, as in <imgref piezo_dovetail>, grip the assembly by the front and back, and pull towards you (while pushing on the right angle bracket). When you re-insert the assembly, it will “click” into position, and then the set screw must be re-tightened.
-<WRAP left round info 50%>
+After the objectives are screwed into the bushings, use the OBLPA adjustment screw and the two objective bushings to move the objectives until they are visually symmetric from the front, as in <imgref two_objectives_closeup>, and visually aligned when viewed from the side of the microscope.
-Notice: If your system uses POMs, take care that you do not unnecessarily apply mechanical stress to the piezos when screwing the objectives into their bushing; they can be permanently damaged.
-</WRAP>\\ \\ \\ \\ \\
-Use the OBLPA adjustment screw and the two objective bushings to move the objectives until they are visually symmetric from the front, as in <imgref two_objectives_closeup>, and visually aligned when viewed from the side of the microscope.
-<imgcaption piezo_dovetail| Side view of SPIM head: set screw for securing the piezo assembly to the SPIM arm and on either side is a screw with ball-nose spring plunger that allows causes the assembly to "click" into place>{{:docs:manual/piezo_dovetail.png?300| Set screw for loosening the piezo assembly from the SPIM arm}} </imgcaption>
+<imgcaption piezo_dovetail| Side view of SPIM head: set screw for securing the piezo assembly to the SPIM arm and on either side is a screw with ball-nose spring plunger that allows causes the assembly to "click" into place>{{:docs:manual/piezo_dovetail.png?300| Set screw for loosening the objective mounting assembly from the SPIM arm}} </imgcaption>
-<imgcaption two_objectives_closeup| Nikon 40x, 0.8 NA, 3.5 mm WD objectives co-focused with ~0.5 mm gap>{{:docs:manual/two_objectives_closeup.jpg?463|Co-focused objectives with ~0.5 mm nominal gap}}</imgcaption>
+<imgcaption two_objectives_closeup| Special Optics ~17x, 0.4 NA, 12 mm WD objectives co-focused with ~12 mm gap>{{:docs:clearedtissueobjectives.jpg?400|Nominal spacing between these objectives is ~12 mm}}</imgcaption>
+<imgcaption ct_objectives| Cleared tissue objectives in dipping media; the silver knob near the right objective is part of the OBLPA which produces lateral movement for the in the Y-axis to facilitate co-focusing the objectives>{{:docs:ct-dspimobjectivesmounted.jpg?400|Cleared tissue objectives mounted to SPIM head}}</imgcaption>
 ===== Cables =====

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