Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision 		Previous revision
Next revision Both sides next revision
docs:mm_dispim_plugin_user_guide [2019/03/08 17:39]
Jon Daniels [Multiple Positions (XY)] 		docs:mm_dispim_plugin_user_guide [2019/07/03 00:03]
Jon Daniels [Acquisition Mode]
Line 171: Line 171:
  
 Currently several modes are supported: Currently several modes are supported:
-  * Synchronous piezo/slice scan: standard use, the piezo and sheet move together through the sample +  * Synchronous piezo/slice scan: standard piezo use, the piezo and sheet move together through the sample 
-  * No scan (fixed sheet): neither the piezo nor light sheet moves; useful for characterizing vibration in the system+  * No scan (fixed sheet): none of the piezo nor light sheet nor the stage moves (only the light sheet generation using the sheet axis); useful for characterizing vibration in the system
   * Stage scan: uses the XY stage to move the sample through a fixed light sheet; for two-sided acquisitions, one view is collected in the first pass, and the second view is collected as the stage moves “back” for a second pass; see the document on stage scanning for details and requirements   * Stage scan: uses the XY stage to move the sample through a fixed light sheet; for two-sided acquisitions, one view is collected in the first pass, and the second view is collected as the stage moves “back” for a second pass; see the document on stage scanning for details and requirements
   * Stage scan interleaved: same as stage scan except that the stage moves half as fast and the two light paths alternate, meaning both views are collected in the same pass; use caution if you are using a galvo to switch the laser between the sides, this can overstress some galvos   * Stage scan interleaved: same as stage scan except that the stage moves half as fast and the two light paths alternate, meaning both views are collected in the same pass; use caution if you are using a galvo to switch the laser between the sides, this can overstress some galvos
-  * Stage scan unidirectional: same as stage scan except the second view is taken while scanning in the same direction as the first view +  * Stage scan unidirectional: same as stage scan except the second view is taken while scanning in the same direction as the first view with a rewind step between.  For 1-sided acquisitions stage scan and stage scan unidirectional are equivalent.  For 2-sided acquisitions the stage scan unidirectional will take slightly longer (the rewind step is done at 75% of the max stage speed) but makes data processing easier. 
-  * Slice scan only: suppresses movement of piezo but moves the light sheet; useful for characterizing light sheet thickness +  * Slice scan only: suppresses movement of piezo but moves the light sheet; useful for characterizing light sheet thickness and for autofocus 
-  * Piezo scan only: the light sheet stays stationary but the piezo moves; useful for establishing axial PSF+  * Piezo scan only: the light sheet stays stationary but the piezo moves; useful for establishing axial PSF and for autofocus
  
  

Trace: