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hardware:computer [2018/10/16 23:32]
Jon Daniels [Specific suggestions]
hardware:computer [2021/02/13 21:28] (current)
Jon Daniels [Acquisition]
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 Otherwise the main requirement having sufficiently fast disk write speed to handle the camera data.  Depending on the use case, solid state drives (SSDs) and/or RAID0 with SSDs may or may not be required.  Individual users should consider their requirements. Otherwise the main requirement having sufficiently fast disk write speed to handle the camera data.  Depending on the use case, solid state drives (SSDs) and/or RAID0 with SSDs may or may not be required.  Individual users should consider their requirements.
  
-The sCMOS cameras used with diSPIM can generate 800 MB/sec (100 fps at 4 MP, 16 bits per pixel).  However the maximum possible frame rate of the camera is not achieved for diSPIM.((Light sheet illumination only occurs during global exposure, and camera-limited frame rates occur without any global exposure time.))  Typical maximum acquisition speeds are 1024x1024 at 50fps or 512x512 at 200 fps; both these situations both generate 100MB/sec.  The average data rate, and hence hard drive speed requirement, is usually even less because most commonly acquisition occurs in bursts (i.e. there is time between successive time points) and a RAM buffer initially holds images so the hard drive needs to keep up with the average data rate.  At present only one camera works at a time, though in the future there are schemes where both cameras could be used simultaneously and thus double the data rate.+The sCMOS cameras used with diSPIM can generate 800 MB/sec (100 fps at 4 MP, 16 bits per pixel).  However the maximum possible frame rate of the camera is not achieved for diSPIM.((Light sheet illumination only occurs during global exposure, and camera-limited frame rates occur without any global exposure time.))  Typical maximum acquisition speeds are 1024x1024 at 50fps or 512x512 at 200 fps; both these situations both generate 100MB/sec.  The average data rate, and hence hard drive speed requirement, is usually even less because most commonly acquisition occurs in bursts (i.e. there is time between successive time points) and a RAM buffer initially holds images so the hard drive needs to keep up with the average data rate.  Usually only one camera works at a time, though there are schemes where both cameras could be used simultaneously and thus double the data rate or else multiple cameras could be used for simultaneous multi-channel recording.
  
-100 MB/sec is typical for a magnetic hard drive.  300 MB/sec is typical for a single SSD.  If the data rate is too high for a single SSD, use SSDs in RAID0 configuration (e.g. 4 SSDs in RAID0 can achieve >1 GB/s).  To benchmark your PC's hard drive write speed you can use [[http://crystalmark.info/?lang=en | Crystal Disk Mark]].  I'm pretty sure the relevant score to diSPIM acquisition is the "Seq" "Write" score (Sequential (Block Size=1MiB) Read/Write with single Thread), at least for Micro-manager software with typical acquisition settings.+100 MB/sec is typical for a magnetic hard drive.  300 MB/sec is typical for a single SSD.  If the data rate is too high for a single SSD, use SSDs in RAID0 configuration (e.g. 4 SSDs in RAID0 can achieve >1 GB/s).  Lately M.2 drives with PCIe interface with comparable speeds to a RAID0 with SSDs have become available and might be a good option.  To benchmark your PC's hard drive write speed you can use [[http://crystalmark.info/?lang=en | Crystal Disk Mark]].  I'm pretty sure the relevant score to diSPIM acquisition is the "Seq" "Write" score (Sequential (Block Size=1MiB) Read/Write with single Thread), at least for Micro-manager software with typical acquisition settings.