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hardware:lasers [2017/08/01 15:36]
Jon Daniels [Getting Dual Outputs] 		hardware:lasers [2022/10/17 22:36]
Steve Saltekoff [Oxxius Laser Combiner]
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 In general the laser power requirements are very modest, e.g. a few mW of optical power at the sample at most and often less than 1 mW.  The exact power required depends on the sample (fluorophore density, efficiency, required SNR and readout speed to get the biological answers you're seeking).  Light sheet microscopy is used precisely because it doesn't need much excitation light, but every sample will bleach eventually so the intensity is often increased to improve SNR until bleaching becomes problematic. In general the laser power requirements are very modest, e.g. a few mW of optical power at the sample at most and often less than 1 mW.  The exact power required depends on the sample (fluorophore density, efficiency, required SNR and readout speed to get the biological answers you're seeking).  Light sheet microscopy is used precisely because it doesn't need much excitation light, but every sample will bleach eventually so the intensity is often increased to improve SNR until bleaching becomes problematic.
 +
 +Often the laser launch is the most expensive part of the light sheet microscope, so if you are looking to save money then start by looking here.  It is possible to assemble your own laser combiner; see e.g. the [[https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0173879|"Nicolase" combiner]].
  
 The ASI scanner has a fiber optic input with either an FC/PC or FC/APC connector (specify which when ordering).  The focal length of the collimation lens should match the fiber's NA to fill the scanning mirror appropriately, for NA ~0.11 the default 12.5 mm focal length lens works well and for NA ~0.06 order the 20 mm focal length option.  Single-mode fiber is required for intended operation. The ASI scanner has a fiber optic input with either an FC/PC or FC/APC connector (specify which when ordering).  The focal length of the collimation lens should match the fiber's NA to fill the scanning mirror appropriately, for NA ~0.11 the default 12.5 mm focal length lens works well and for NA ~0.06 order the 20 mm focal length option.  Single-mode fiber is required for intended operation.
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 ==== Spectral Applied ILE ==== ==== Spectral Applied ILE ====
  
-(no info seems to be available online but similar to LLM5 with dual output option)+(no info seems to be available online but similar to LLM5 with dual output option; Spectral Applied was purchased by Andor but I think the launch is still available from them)
  
 === Implementation notes === === Implementation notes ===
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   * Probably would work if you had way to control intensity, e.g. by DAC card.   * Probably would work if you had way to control intensity, e.g. by DAC card.
  
 +
 +==== Oxxius Laser Combiner ====
 +
 +=== Implementation notes ===
 +  * Dual output available
 +  * Many features and wavelengths available
 +
 +=== Usage ===
 +  * Make sure you place the fiber coupler on its correct magnetic base. Otherwise you can have significant power loss.
 +=== Micro-manager notes ===
 +  * The combiner must be set up as a virtual comm port. Use the "CDC" command. (https://micro-manager.org/Oxxius_combiner) 
 +  *It is recommended that you include, emission, shutters, and digital modulation in the startup group.

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