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hardware:objectives [2023/08/08 22:36]
Jon Daniels [Close-up Drawings] |
hardware:objectives [2024/03/07 00:37]
Jon Daniels [Mechanical Angle] |
| ==== Magnification ==== | ==== Magnification ==== |
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| Like all infinity microscopes, the magnification is given by the ratio of the effective focal lengths of tube lens and objective. The captured fField of view is just camera sensor size divided by magnification. The sensor size is readily available. The most common sCMOS cameras have 6.5um square pixels and 2048x2048 pixels, but there are a wide variety of cameras. Some cameras have 11um pixels which usually require increasing magnification in order to sample sufficiently for diffraction-limited resolution. | Like all infinity microscopes, the magnification is given by the ratio of the effective focal lengths of tube lens and objective. The captured field of view is just camera sensor size divided by magnification. The sensor size is readily available. The most common sCMOS cameras have 6.5um square pixels and 2048x2048 pixels, but there are a wide variety of cameras. Some cameras have 11um pixels which usually require increasing magnification in order to sample sufficiently for diffraction-limited resolution. |
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| By default ASI uses 200 mm focal length tube lenses (Nikon glass) but a offers a [[http://asiimaging.com/docs/mim_ramm_vts#mim_tube_lenses_and_assemblies|variety of tube lenses]] so the magnification can easily be chosen. Typical reasons to adjust the magnification include to adequately sample on the camera (for sensors with larger pixels or using low-mag high-NA objectives) or to increase the field of view. When decreasing magnification, beware of the objective's intrinsic field of view (usually specified by field number, of vignetting (see http://asiimaging.com/docs/mim_ramm_vts#infinity_space_limitations), and ensure that the spatial sampling on the camera is sufficient (see https://asiimaging.com/docs/infinity_microscope_basics#spatial_sampling). | By default ASI uses 200 mm focal length tube lenses (Nikon glass) but a offers a [[http://asiimaging.com/docs/mim_ramm_vts#mim_tube_lenses_and_assemblies|variety of tube lenses]] so the magnification can easily be chosen. Typical reasons to adjust the magnification include to adequately sample on the camera (for sensors with larger pixels or using low-mag high-NA objectives) or to increase the field of view. When decreasing magnification, beware of the objective's intrinsic field of view (usually specified by field number, of vignetting (see http://asiimaging.com/docs/mim_ramm_vts#infinity_space_limitations), and ensure that the spatial sampling on the camera is sufficient (see https://asiimaging.com/docs/infinity_microscope_basics#spatial_sampling). |
| | Olympus 60x/1.0 W | 49° | 53° | sometimes oSPIM detection | | | Olympus 60x/1.0 W | 49° | 53° | sometimes oSPIM detection | |
| | Olympus 60x/1.1 W | 56° | 57° | usual oSPIM detection, potential lattice detection | | | Olympus 60x/1.1 W | 56° | 57° | usual oSPIM detection, potential lattice detection | |
| | | Nikon 60x/1.0 W | 49° | 57° | | |
| | SO 54-10-7 29x/0.66 W | 30° | 30° | traditional lattice illumination | | | SO 54-10-7 29x/0.66 W | 30° | 30° | traditional lattice illumination | |
| | TL20X-MPL 20x/0.6 W | 27° | ~30° | new (2020) cost-effective lattice illumination | | | TL20X-MPL 20x/0.6 W | 27° | ~30° | new (2020) cost-effective lattice illumination, only 7mm FN?! | |
| | Nikon 20x/1.0 glyc | 43° | ~54° | cleared tissue confocal used in light sheet (RI 1.44 - 1.50) | | | Nikon 20x/1.0 glyc | 43° | ~54° | cleared tissue confocal used in light sheet (RI 1.44 - 1.50) | |
| | Olympus 25x/1.0 glyc | 43° | 56° | cleared tissue confocal used in light sheet (RI 1.41 - 1.52) | | | Olympus 25x/1.0 glyc | 43° | 56° | cleared tissue confocal used in light sheet (RI 1.41 - 1.52) | |
