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hardware:objectives [2024/03/07 00:37] Jon Daniels [Mechanical Angle] |
hardware:objectives [2024/05/01 09:27] Jon Daniels [Mechanical Angle] |
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ASI and Special Optics have co-developed two [[http:// | ASI and Special Optics have co-developed two [[http:// | ||
- | Single-sided systems (iSPIM) have much more flexibility because the illumination objective can be a low-NA long-WD objective. A popular pair for high-resolution imaging is the same objective pair as used on the lattice light sheet, specifically the Nikon 25x/1.1 objective paired with Special Optics 54-10-7 which is 28.6x/0.66. | + | Single-sided systems (iSPIM) have much more flexibility because the illumination objective can be a low-NA long-WD objective. A popular pair for high-resolution imaging is the same objective pair as used on the lattice light sheet, specifically the Nikon 25x/1.1 objective paired with either the Special Optics 54-10-7 which is 28.6x/0.66, or else paired with the Thorlabs 20x/0.6. |
==== Close-up Drawings ==== | ==== Close-up Drawings ==== | ||
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| Nikon 60x/1.0 W | | Nikon 60x/1.0 W | ||
| SO 54-10-7 29x/0.66 W | | SO 54-10-7 29x/0.66 W | ||
- | | TL20X-MPL 20x/0.6 W | + | | TL20X-MPL 20x/0.6 W |
| Nikon 20x/1.0 glyc | 43° | ~54° | cleared tissue confocal used in light sheet (RI 1.44 - 1.50) | | | Nikon 20x/1.0 glyc | 43° | ~54° | cleared tissue confocal used in light sheet (RI 1.44 - 1.50) | | ||
| Olympus 25x/1.0 glyc | 43° | 56° | cleared tissue confocal used in light sheet (RI 1.41 - 1.52) | | | Olympus 25x/1.0 glyc | 43° | 56° | cleared tissue confocal used in light sheet (RI 1.41 - 1.52) | |