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hardware:objectives [2024/10/23 20:46]
Jon Daniels [Mechanical Angle] 		hardware:objectives [2026/07/16 17:55] (current)
Jon Daniels [Mechanical Angle]
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 | Nikon 16x/0.8 W         |  37°                |  45°              | does it actually fit with pair? (if you try it please speak up)  | | Nikon 16x/0.8 W         |  37°                |  45°              | does it actually fit with pair? (if you try it please speak up)  |
 | Olympus 20x/0.5 W       |  22°                |  45°              | fits better than expected (WD is probably a bit more than spec)  | | Olympus 20x/0.5 W       |  22°                |  45°              | fits better than expected (WD is probably a bit more than spec)  |
-| Olympus 20x/1.0 W       |  49°                |  52°              |   |+| Olympus 20x/1.0 W       |  49°                |  52°              | indications that it fails to achieve diffraction-limited performance  |
 | Nikon 20x/1.0 W         |  49°                |  53°              |                                           | | Nikon 20x/1.0 W         |  49°                |  53°              |                                           |
 | Leica 20x/1.0 W         |  49°                |  52°              |                                           | | Leica 20x/1.0 W         |  49°                |  52°              |                                           |
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 | Nikon 20x/1.0 glyc      |  43°                |  ~54°             | cleared tissue confocal used in light sheet (RI 1.44 - 1.50)     | | Nikon 20x/1.0 glyc      |  43°                |  ~54°             | cleared tissue confocal used in light sheet (RI 1.44 - 1.50)     |
 | Olympus 25x/1.0 glyc    |  43°                |  56°              | cleared tissue confocal used in light sheet (RI 1.41 - 1.52)     | | Olympus 25x/1.0 glyc    |  43°                |  56°              | cleared tissue confocal used in light sheet (RI 1.41 - 1.52)     |
-| SO 54-10-12 17x/0.4 MI  |  16°                |  22°              | ASI multi-immersion #1 (RI 1.33 - 1.56, nominal 1.45)            | +| SO 54-10-12 17x/0.4 MI  |  16°                |  32°              | ASI multi-immersion #1 (RI 1.33 - 1.56, nominal 1.45), mechanical limitation in body   | 
-| SO 54-12-8 24x/0.7 MI   |  29°                |  36°              | ASI multi-immersion #2 (RI 1.33 - 1.56, nominal 1.45)            | +| SO 54-12-8 24x/0.7 MI   |  29°                |  36°              | ASI multi-immersion #2 (RI 1.33 - 1.56, nominal 1.45)   | 
-| SO 54-9-4 52x/1.15 MI   |  52°                |  57°              | (preliminary) ASI multi-immersion #3 (RI 1.33 - 1.56, nominal 1.45)            |+| SO 57-12-19 10x/0.3 MI  |  11°                |  28°              | ASI multi-immersion #3 (RI 1.33 - 1.56, nominal 1.53), mechanical limitation in body  | 
 +| SO 57-12-5 40x/1.2 MI   |  52°                |  59°              | ASI multi-immersion #4 (RI 1.33 - 1.56, nominal 1.52)            | 
 +| SO 57-15-8 27x/1.0 W    |  49°                |  53°              | ASI expansion #1             | 
 + 
 + 
 +==== Working Distance and Imaging Depth ==== 
 + 
 +The imaging depth of a pair of co-focused objectives into a flat pancake-like sample without hitting the top of the sample is proportional to the residual mechanical angle that the two objectives leave and also proportional to their working distance, with minor factor related to the relative mechanical angle/overall rotation.  There may also be second-order mechanical effects and such combinations should be modeled.  ASI has drawings of many different objective pairs and can prepare others.  Please inquire.
  
  

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