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hardware:objectives [2024/10/23 20:46]
Jon Daniels [Mechanical Angle] 		hardware:objectives [2024/11/01 22:02] (current)
Jon Daniels [Mechanical Angle]
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 | Nikon 20x/1.0 glyc      |  43°                |  ~54°             | cleared tissue confocal used in light sheet (RI 1.44 - 1.50)     | | Nikon 20x/1.0 glyc      |  43°                |  ~54°             | cleared tissue confocal used in light sheet (RI 1.44 - 1.50)     |
 | Olympus 25x/1.0 glyc    |  43°                |  56°              | cleared tissue confocal used in light sheet (RI 1.41 - 1.52)     | | Olympus 25x/1.0 glyc    |  43°                |  56°              | cleared tissue confocal used in light sheet (RI 1.41 - 1.52)     |
-| SO 54-10-12 17x/0.4 MI  |  16°                |  22°              | ASI multi-immersion #1 (RI 1.33 - 1.56, nominal 1.45)            | +| SO 54-10-12 17x/0.4 MI  |  16°                |  32°              | ASI multi-immersion #1 (RI 1.33 - 1.56, nominal 1.45), mechanical limitation in body   | 
-| SO 54-12-8 24x/0.7 MI   |  29°                |  36°              | ASI multi-immersion #2 (RI 1.33 - 1.56, nominal 1.45)            |+| SO 54-12-8 24x/0.7 MI   |  29°                |  36°              | ASI multi-immersion #2 (RI 1.33 - 1.56, nominal 1.45)   | 
 +| SO 57-12-19 10x/0.3 MI  |  11°                |  28°              | ASI multi-immersion #3 (RI 1.33 - 1.56, nominal 1.45), mechanical limitation in body  |
 | SO 54-9-4 52x/1.15 MI   |  52°                |  57°              | (preliminary) ASI multi-immersion #3 (RI 1.33 - 1.56, nominal 1.45)            | | SO 54-9-4 52x/1.15 MI   |  52°                |  57°              | (preliminary) ASI multi-immersion #3 (RI 1.33 - 1.56, nominal 1.45)            |
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 +
 +==== Working Distance and Imaging Depth ====
 +
 +The imaging depth of a pair of co-focused objectives into a flat pancake-like sample without hitting the top of the sample is proportional to the residual mechanical angle that the two objectives leave and also proportional to their working distance, with minor factor related to the relative mechanical angle/overall rotation.  There may also be second-order mechanical effects and such combinations should be modeled.  ASI has drawings of many different objective pairs and can prepare others.  Please inquire.
  
  

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