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hardware:objectives [2024/02/22 23:46]
Jon Daniels [Magnification] 		hardware:objectives [2024/05/01 09:27] (current)
Jon Daniels [Mechanical Angle]
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 ASI and Special Optics have co-developed two [[http://asiimaging.com/docs/cleared_tissue_objective|multi-immersion objectives]] designed originally for cleared tissue (but useful in any media) suitable for the diSPIM geometry.  The 54-10-12 with nominal NA 0.4 can image cleared tissue up to 5 mm deep in slab form or within a 12 mm spherical envelope, and the 54-12-8 with nominal NA 0.7 can go 2 mm deep or 10 mm spherical envelope. ASI and Special Optics have co-developed two [[http://asiimaging.com/docs/cleared_tissue_objective|multi-immersion objectives]] designed originally for cleared tissue (but useful in any media) suitable for the diSPIM geometry.  The 54-10-12 with nominal NA 0.4 can image cleared tissue up to 5 mm deep in slab form or within a 12 mm spherical envelope, and the 54-12-8 with nominal NA 0.7 can go 2 mm deep or 10 mm spherical envelope.
  
-Single-sided systems (iSPIM) have much more flexibility because the illumination objective can be a low-NA long-WD objective. A popular pair for high-resolution imaging is the same objective pair as used on the lattice light sheet, specifically the Nikon 25x/1.1 objective paired with Special Optics 54-10-7 which is 28.6x/0.66.+Single-sided systems (iSPIM) have much more flexibility because the illumination objective can be a low-NA long-WD objective. A popular pair for high-resolution imaging is the same objective pair as used on the lattice light sheet, specifically the Nikon 25x/1.1 objective paired with either the Special Optics 54-10-7 which is 28.6x/0.66, or else paired with the Thorlabs 20x/0.6.
  
 ==== Close-up Drawings ==== ==== Close-up Drawings ====
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 | Nikon 60x/1.0 W         |  49°                |  57°              |            | | Nikon 60x/1.0 W         |  49°                |  57°              |            |
 | SO 54-10-7 29x/0.66 W   |  30°                |  30°              | traditional lattice illumination                                 | | SO 54-10-7 29x/0.66 W   |  30°                |  30°              | traditional lattice illumination                                 |
-| TL20X-MPL 20x/0.6 W     |  27°                |  ~30°             | new (2020) cost-effective lattice illumination                   |+| TL20X-MPL 20x/0.6 W     |  27°                |  ~30°             | new (2020) cost-effective lattice illumination, note only 7mm FN at full NA    |
 | Nikon 20x/1.0 glyc      |  43°                |  ~54°             | cleared tissue confocal used in light sheet (RI 1.44 - 1.50)     | | Nikon 20x/1.0 glyc      |  43°                |  ~54°             | cleared tissue confocal used in light sheet (RI 1.44 - 1.50)     |
 | Olympus 25x/1.0 glyc    |  43°                |  56°              | cleared tissue confocal used in light sheet (RI 1.41 - 1.52)     | | Olympus 25x/1.0 glyc    |  43°                |  56°              | cleared tissue confocal used in light sheet (RI 1.41 - 1.52)     |

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