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hardware:troubleshooting [2015/05/15 18:42] Jon Daniels created |
hardware:troubleshooting [2018/05/04 00:21] Jon Daniels [Vibration] |
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===== Troubleshooting ===== | ===== Troubleshooting ===== | ||
- | ^ Problem | + | Try to isolate the problem to the extent possible. |
+ | |||
+ | ^ Problem | ||
+ | | Communication error between software and hardware | | Power cycle the affected hardware and restart the software | | ||
+ | | One hardware element not responding | | Connect to the hardware using manufacturer' | ||
| Beams only come out of the objective if both beams are turned on | Fibers going to the wrong scanner | Exchange the fibers | | | Beams only come out of the objective if both beams are turned on | Fibers going to the wrong scanner | Exchange the fibers | | ||
| Stage scanning cameras not getting triggered | Hardware/ | | Stage scanning cameras not getting triggered | Hardware/ | ||
+ | | Acquisition images look different than alignment (look like epi view) | Camera trigger cables swapped | Swap the camera trigger cables | | ||
+ | | Inconsistent communication with Tiger controller | Windows comm port problems | Update USB to comm port [[http:// | ||
+ | |||
+ | ==== Piezos ==== | ||
+ | |||
+ | The piezo objective movers are the most fragile and failure-prone component of the diSPIM. | ||
+ | |||
+ | ==== Software ==== | ||
+ | |||
+ | If you encounter bugs in the software it is best to contact the developers directly. | ||
+ | |||
+ | ==== Vibration ==== | ||
+ | |||
+ | Some users have reported vibrations, e.g. diffraction-size beads will appear as a diagonal smear. | ||
+ | |||
+ | There are three approaches to reduce the apparent vibration: | ||
+ | |||
+ | - **Reduce camera vibration: | ||
+ | - **Mechanically decouple the camera and piezo:** You can mount the camera to the air table instead of the the microscope. | ||
+ | - **Reduce susceptibility of piezo to vibrate:** If you have an 2014 or older ASI piezo objective mover you can update to the version introduced early 2015 which is significantly stiffer and hence less prone to vibration. |