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hardware:troubleshooting [2016/02/16 20:02]
Jon Daniels [Vibration]
hardware:troubleshooting [2018/05/04 00:21]
Jon Daniels [Vibration]
Line 9: Line 9:
 | Stage scanning cameras not getting triggered | Hardware/firmware out of date | Make sure you have Rev F XY card or later, make sure micro-mirror card has jumper on positions 11/12 of SV6 | | Stage scanning cameras not getting triggered | Hardware/firmware out of date | Make sure you have Rev F XY card or later, make sure micro-mirror card has jumper on positions 11/12 of SV6 |
 | Acquisition images look different than alignment (look like epi view) | Camera trigger cables swapped | Swap the camera trigger cables | | Acquisition images look different than alignment (look like epi view) | Camera trigger cables swapped | Swap the camera trigger cables |
-| Inconsistent communication with Tiger controller | Windows comm port problems | Update USB to comm port [[http://www.silabs.com/products/mcu/Pages/USBtoUARTBridgeVCPDrivers.aspx|driver]], disable USB suspend in Windows per [[https://micro-manager.org/wiki/Personal_computer_setup|Micro-manager recommendations]] |+| Inconsistent communication with Tiger controller | Windows comm port problems | Update USB to comm port [[http://www.silabs.com/products/mcu/Pages/USBtoUARTBridgeVCPDrivers.aspx|driver (NOT universal version)]], disable USB suspend in Windows per [[https://micro-manager.org/wiki/Personal_computer_setup|Micro-manager recommendations]] |
  
 ==== Piezos ==== ==== Piezos ====
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 ==== Vibration ==== ==== Vibration ====
  
-Some users have reported vibrations, e.g. diffraction-size beads will appear as a diagonal smear.  Assuming you have the system on a floated air table, this is probably due to a combination of the vibration of the camera fan and the vibration-prone nature of ASI's original piezo objective movers.  Some cameras vibrate that others, even within the same brand/type there seems to be a large variability.  PCO cameras seemed to be the worst offenders until mid-2015.+Some users have reported vibrations, e.g. diffraction-size beads will appear as a diagonal smear.  Assuming you have the system on a floated air table, this is probably due to a combination of the vibration of the camera fan and the "vibration-ability" of the piezo objective movers. The exact threshold of when vibrations become noticeable depends on the experiment specifics.
  
 There are three approaches to reduce the apparent vibration: There are three approaches to reduce the apparent vibration:
    
-  - **Reduce camera vibration:** Try swapping cameras, even with another one that is nominally identical, to see if the problem is reduced.  Some cameras can be water-cooled in which case the fan can be turned off, e.g. Hamamatsu Flash4.  Some cameras can turn their fans off, e.g. Andor Zyla (only recommended for bursts of acquisition, e.g. if you acquire for a few seconds every minute). +  - **Reduce camera vibration:** Try swapping cameras, even with another one that is nominally identical, to see if the problem is reduced; there can be significant variability even within the same brand/type of camera.  Some cameras can be water-cooled in which case the fan can be turned off, e.g. Hamamatsu Flash4.  Some cameras can turn their fans off, e.g. Andor Zyla (only recommended for bursts of acquisition, e.g. if you acquire for a few seconds every minute).  PCO Edge cameras seemed to have the worst vibration initially, but in mid-2015 their internal design was modified to correct this and now all three major camera brands seem roughly comparable. 
-  +  - **Mechanically decouple the camera and piezo:** You can mount the camera to the air table instead of the the microscope.   As of mid-2016 ASI offers a universal air table mount for this purpose, and several groups have rigged this up themselves.  There is some evidence that the CDZ-R block used on RAMM-mounted diSPIM reduces the vibration compared with the CDZ-1000 used when the diSPIM is mounted on other inverted microscopes
-  - **Mechanically decouple the camera and piezo:** You can mount the camera to the air table instead of the the microscope.  Several groups have rigged this up themselves.  In early 2016 ASI designed a universal air table mount for this purpose; it is still being prototyped but having an anxious customer would expedite things. +  - **Reduce susceptibility of piezo to vibrate:** If you have an 2014 or older ASI piezo objective mover you can update to the version introduced early 2015 which is significantly stiffer and hence less prone to vibration.  At the same time the mounting scheme was changed which makes alignment significantly easier and offers other minor benefits.  The only downside to updating the piezo is that the travel range will be 150um instead of 300um.  ASI performs such updates at cost, contact them for details.  If you are only doing stage scanning the piezos can be eliminated entirely.
- +
-  - **Reduce susceptibility of piezo to vibrate:** You can update the piezo objective mover to the version introduced early 2015 which is significantly stiffer.  At the same time the scheme was changed for mounting the piezo to the SPIM arm which makes alignment significantly easier and offers some other minor benefits.  The only downside to updating the piezo is that the travel range will be 200um instead of 300um.  ASI performs such updates at cost but it is not free.+