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hardware:troubleshooting [2019/01/22 18:02]
Melissa Glidewell added section "Out of Bushing Travel" |
hardware:troubleshooting [2022/04/05 11:54]
Jon Daniels [Vibration] |
| - **Reduce camera vibration:** Try swapping cameras, even with another one that is nominally identical, to see if the problem is reduced; there can be significant variability even within the same brand/type of camera. Some cameras can be water-cooled in which case the fan can be turned off, e.g. Hamamatsu Flash4. Some cameras can turn their fans off, e.g. Andor Zyla (only recommended for bursts of acquisition, e.g. if you acquire for a few seconds every minute). PCO.edge cameras seemed to have the worst vibration initially, but in mid-2015 their internal design was modified to correct this and now all three major camera brands seem roughly comparable. | - **Reduce camera vibration:** Try swapping cameras, even with another one that is nominally identical, to see if the problem is reduced; there can be significant variability even within the same brand/type of camera. Some cameras can be water-cooled in which case the fan can be turned off, e.g. Hamamatsu Flash4. Some cameras can turn their fans off, e.g. Andor Zyla (only recommended for bursts of acquisition, e.g. if you acquire for a few seconds every minute). PCO.edge cameras seemed to have the worst vibration initially, but in mid-2015 their internal design was modified to correct this and now all three major camera brands seem roughly comparable. |
| - **Mechanically decouple the camera and piezo:** You can mount the camera to the air table instead of the the microscope. As of mid-2016 ASI offers a universal air table mount for this purpose, and several groups have rigged this up themselves. There is some evidence that the CDZ-R block used on RAMM-mounted diSPIM reduces the vibration compared with the CDZ-1000 used when the diSPIM is mounted on other inverted microscopes. | - **Mechanically decouple the camera and piezo:** You can mount the camera to the air table instead of the the microscope. As of mid-2016 ASI offers a universal air table mount for this purpose, and several groups have rigged this up themselves. There is some evidence that the CDZ-R block used on RAMM-mounted diSPIM reduces the vibration compared with the CDZ-1000 used when the diSPIM is mounted on other inverted microscopes. |
| - **Reduce susceptibility of piezo to vibrate:** If you have an 2014 or older ASI piezo objective mover you can update to the version introduced early 2015 which is significantly stiffer and hence less prone to vibration. At the same time the mounting scheme was changed which makes alignment significantly easier and offers other minor benefits. The only downside to updating the piezo is that the travel range will be 150 um instead of 300 um. ASI performs such updates at cost, contact them for details. If you are only doing stage scanning the piezos can be eliminated entirely. | - **Reduce susceptibility of piezo to vibrate:** If you have an 2014 or older ASI piezo objective mover you can update to the version introduced early 2015 which is significantly stiffer and hence less prone to vibration. At the same time the mounting scheme was changed which makes alignment significantly easier and offers other minor benefits. The only downside to updating the piezo is that the travel range will be 150 um instead of 300 um. ASI performs such updates at cost, contact them for details. If you are only doing stage scanning the piezos can be eliminated entirely. As of late 2021 ASI offers a bulkier and more expensive piezo objective mover ("s-POM") which is significantly less susceptible to vibration than the standard "f-POM". |
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| ==== Out of Bushing Travel ==== | ==== Out of Bushing Travel ==== |
