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software:micro-manager [2024/04/15 23:19] Jon Daniels [Micro-manager diSPIM Plugin] |
software:micro-manager [2024/05/21 23:52] (current) Jon Daniels [Runnables in the plugin] |
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Line 431: | Line 431: | ||
diSPIM.attachRunnable(taskStartTimepoint, | diSPIM.attachRunnable(taskStartTimepoint, | ||
diSPIM.attachRunnable(taskEndTimepoint, | diSPIM.attachRunnable(taskEndTimepoint, | ||
+ | |||
+ | </ | ||
+ | |||
+ | |||
+ | Here is another example that runs autofocus periodically, | ||
+ | |||
+ | < | ||
+ | |||
+ | // runs autofocus once on the first position and periodically afterwards based on period set in script (currently every 13th position) | ||
+ | // script assumes that CRISP is already set up and is the default focus device | ||
+ | // run this script after launching the plugin and before beginning the acquisition | ||
+ | |||
+ | // required imports | ||
+ | import org.micromanager.asidispim.api.*; | ||
+ | |||
+ | // get a reference to the plugin; use this to call the plugins API methods | ||
+ | ASIdiSPIMInterface diSPIM = new ASIdiSPIMImplementation(); | ||
+ | |||
+ | // local variables | ||
+ | int runnablePositionCounter; | ||
+ | int counterPeriod = 13; | ||
+ | |||
+ | // initialize the counter when the acquisition begins | ||
+ | taskStartAcquisition = new Runnable() { | ||
+ | public void run() { | ||
+ | runnablePositionCounter = 0; | ||
+ | } | ||
+ | }; | ||
+ | |||
+ | // at each position see if it's time to run autofocus, plus also increment the counter | ||
+ | taskStartPosition = new Runnable() { | ||
+ | public void run() { | ||
+ | // | ||
+ | if ((runnablePositionCounter % counterPeriod)< | ||
+ | mmc.fullFocus(); | ||
+ | } | ||
+ | runnablePositionCounter++; | ||
+ | } | ||
+ | }; | ||
+ | |||
+ | |||
+ | // add the runnables to the diSPIM plugin' | ||
+ | diSPIM.clearAllRunnables(); | ||
+ | diSPIM.attachRunnable(taskStartAcquisition, | ||
+ | diSPIM.attachRunnable(taskStartPosition, | ||
+ | |||
</ | </ |