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start [2017/07/20 19:56]
Jon Daniels [The Implementation] |
start [2017/08/22 16:58]
Jon Daniels [The Implementation] |
| ===== The Implementation ===== | ===== The Implementation ===== |
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| Two objectives are placed at right angles above a sample mounted horizontally in an open dish, each objective 45 degrees from vertical. A light sheet is created from one objective and imaged using the other objective. A stack of images is collected by moving the light sheet through the sample. For a few users, the 3D information from a single view or stack is sufficient (iSPIM). For dual-view systems, the role of the two objectives is reversed to collect another stack from a perpendicular direction, and then the two datasets can be computationally merged to yield a 3D dataset with isotropic resolution (the usual problem of poor axial resolution is overcome by information from the other view). The dual-view diSPIM thus has two (usually symmetric) optical paths including two scanners and two cameras. | Two objectives are placed at right angles above a sample mounted horizontally in an open dish, each objective 45 degrees from vertical. A light sheet is created from one objective and imaged using the other objective. A stack of images is collected by moving the light sheet through the sample. For some applications the 3D information from a single view or stack is sufficient (iSPIM). For dual-view systems, the role of the two objectives is reversed to collect another stack from a perpendicular direction, and then the two datasets can be computationally merged to yield a 3D dataset with isotropic resolution (the usual problem of poor axial resolution is overcome by information from the other view). The dual-view diSPIM thus has two (usually symmetric) optical paths including two scanners and two cameras. iSPIM is a subset of diSPIM with a single camera and scanner but otherwise nearly identical. |
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| {{ :dispim_on_ramm.jpg?direct&250|}} | {{ :dispim_on_ramm.jpg?direct&250|}} |
