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start [2023/02/11 20:35]
Jon Daniels [The Implementation]
start [2024/05/01 09:37] (current)
Jon Daniels [The Implementation]
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 ===== The Implementation ===== ===== The Implementation =====
  
-Two objectives are placed at right angles above a sample mounted horizontally in an open dish, each objective 45 degrees from vertical.  A light sheet is created from one objective and imaged using the other objective. A stack of images is collected by moving the light sheet through the sample.  For some applications the 3D information from a single view or stack is sufficient (iSPIM).  For dual-view systems, the role of the two objectives is reversed to collect another stack from a perpendicular direction, and then the two datasets can be computationally merged to yield a 3D dataset with isotropic resolution (the usual problem of poor axial resolution is overcome by information from the other view).  The dual-view diSPIM thus has two (usually symmetric) optical paths including two scanners and two cameras.  iSPIM is a subset of diSPIM with a single camera and scanner but otherwise nearly identical.+Two objectives are placed at right angles above a sample mounted horizontally in an open dish, usually each objective 45 degrees from vertical.  A light sheet is created from one objective and imaged using the other objective. A stack of images is collected by moving the light sheet through the sample.  For some applications the 3D information from a single view or stack is sufficient (iSPIM).  For dual-view systems, the role of the two objectives is reversed to collect another stack from a perpendicular direction, and then the two datasets can be computationally merged to yield a 3D dataset with isotropic resolution (the usual problem of poor axial resolution is overcome by information from the other view).  The dual-view diSPIM thus has two (usually symmetric) optical paths including two scanners and two cameras.  iSPIM is a subset of diSPIM with a single camera and scanner but otherwise nearly identical.
  
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