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docs:comparison_with_confocal [2015/06/15 21:17] Jon Daniels created |
docs:comparison_with_confocal [2015/08/06 20:04] Jon Daniels |
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===== Comparison of diSPIM With Confocal ===== | ===== Comparison of diSPIM With Confocal ===== | ||
- | | + | The following comparison was written with diSPIM in mind, but the main points apply to all types of SPIM/ |
- | * In general for X slices | + | |
- | * [[http:// | + | Spinning disk confocal (SDCM) is just a massively parallel confocal implementation so it's faster but otherwise has the some characteristics as laser scanning confocal. |
- | * Confocal | + | |
- | | + | * **Light dose:** Confocal has much more photobleaching/ |
- | * diSPIM is comparable speed (per view) to to spinning disk confocal | + | * A rule of thumb is that you need X times as much light for confocal as you do for SPIM where X is the number of slices in your stack. |
+ | * [[http:// | ||
+ | * **Z resolution: | ||
+ | * Using Bessel beams to create the sheet can improve Z-resolution for single-view SPIM, but won't meaningfully change dual view resolution | ||
+ | * **XY resolution:** confocal | ||
+ | * **Speed:** SPIM (per view) and SDCM have comparable speed (assuming | ||
+ | * Camera readout bounds the maximum achievable frame rate. For example, 512 pixels high ROI is 2.5 ms readout time for sCMOS. |