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docs:manual:alignment [2015/06/26 17:38] Jon Daniels finished adding from lyx |
docs:manual:alignment [2020/08/18 14:45] Jon Daniels [Beam position and angle: what affects what?] |
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===== Beam position and angle: what affects what? ===== | ===== Beam position and angle: what affects what? ===== | ||
- | We are concerned with getting both the beam position and angle correct in the sample. Fortunately, | + | We are concerned with getting both the beam position and angle correct in the sample, plus making sure the objectives are looking at the same place. Fortunately, |
< | < | ||
^ Adjustment | ^ Adjustment | ||
- | | Tilt of dichroic mirror | + | | Tilt of dichroic mirror |
which the beam enters the back focal plane as well as the beam position at the back focal plane. It is possible to | which the beam enters the back focal plane as well as the beam position at the back focal plane. It is possible to | ||
compensate by adjusting the scan center but we usually adjust the camera mirrors to compensate instead.)) | | compensate by adjusting the scan center but we usually adjust the camera mirrors to compensate instead.)) | | ||
- | | Tilt of camera mirror | + | | Tilt of camera mirror |
- | | Position of upper Z stage | + | | Z position of SPIM head relative to sample |
- | | Tilt of adjustable mirror | + | | Tilt of adjustable mirror |
- | | Tilt of main micro-mirror | + | | Tilt of main micro-mirror |
</ | </ | ||