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docs:mm_dispim_plugin_user_guide [2019/03/08 17:39]
Jon Daniels [Multiple Positions (XY)] 		docs:mm_dispim_plugin_user_guide [2019/05/30 09:12]
Jon Daniels [Acquisition Mode]
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   * Stage scan: uses the XY stage to move the sample through a fixed light sheet; for two-sided acquisitions, one view is collected in the first pass, and the second view is collected as the stage moves “back” for a second pass; see the document on stage scanning for details and requirements   * Stage scan: uses the XY stage to move the sample through a fixed light sheet; for two-sided acquisitions, one view is collected in the first pass, and the second view is collected as the stage moves “back” for a second pass; see the document on stage scanning for details and requirements
   * Stage scan interleaved: same as stage scan except that the stage moves half as fast and the two light paths alternate, meaning both views are collected in the same pass; use caution if you are using a galvo to switch the laser between the sides, this can overstress some galvos   * Stage scan interleaved: same as stage scan except that the stage moves half as fast and the two light paths alternate, meaning both views are collected in the same pass; use caution if you are using a galvo to switch the laser between the sides, this can overstress some galvos
-  * Stage scan unidirectional: same as stage scan except the second view is taken while scanning in the same direction as the first view+  * Stage scan unidirectional: same as stage scan except the second view is taken while scanning in the same direction as the first view with a rewind step between.  For 1-sided acquisitions stage scan and stage scan unidirectional are equivalent.  For 2-sided acquisitions the stage scan unidirectional will take slightly longer (the rewind step is done at 75% of the max stage speed) but makes data processing easier.
   * Slice scan only: suppresses movement of piezo but moves the light sheet; useful for characterizing light sheet thickness   * Slice scan only: suppresses movement of piezo but moves the light sheet; useful for characterizing light sheet thickness
   * Piezo scan only: the light sheet stays stationary but the piezo moves; useful for establishing axial PSF   * Piezo scan only: the light sheet stays stationary but the piezo moves; useful for establishing axial PSF

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