oSpim

oSPIM stands for Oblique SPIM. The light sheet is generated using an objective below the sample, usually an oil objective, and is tilted partway towards TIRF. The imaging objective is a water dipping objective that is lowered into the media. The oSPIM is an ideal tool for cell biologists or other applications where the sample is in close contact with the coverslip. The same concept was independently and concurrently developed by a research group in Heidelberg and called the πSPIM (see 10.1038/srep32880|their publication). For more details about oSPIM see the presentation given in December 2016 at ASCB.

There is a Micro-Manager plugin for the oSPIM, based heavily on the diSPIM plugin but with a few changes. In the future hopefully the oSPIM vs. diSPIM will hopefully be a setting changed by the user in the plugin, but for now there is a flag in the source code that needs to be changed and the code be rebuilt.

Start by installing a recent nightly build of Micro-Manager 1.4.x. The latest official build is quite old at this point, so make sure to grab a nightly build. For Windows the nightly builds are here.

Download the latest copy of the plugin here (last update 22-Jun-2017) or else one from the archives. Make sure Micro-Manager is not running and then copy the JAR file into C:\Program Files\Micro-Manager-1.4\mmplugins (it will take precedence over the ASIdiSPIM plugin in the Device_Control folder, or you can delete ASIdiSPIM.jar from that folder and replace it with this one).