Differences
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hardware:troubleshooting [2015/05/20 00:00] Jon Daniels |
hardware:troubleshooting [2016/02/17 17:40] Jon Daniels [Vibration] |
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| Beams only come out of the objective if both beams are turned on | Fibers going to the wrong scanner | Exchange the fibers | | | Beams only come out of the objective if both beams are turned on | Fibers going to the wrong scanner | Exchange the fibers | | ||
| Stage scanning cameras not getting triggered | Hardware/ | | Stage scanning cameras not getting triggered | Hardware/ | ||
+ | | Acquisition images look different than alignment (look like epi view) | Camera trigger cables swapped | Swap the camera trigger cables | | ||
+ | | Inconsistent communication with Tiger controller | Windows comm port problems | Update USB to comm port [[http:// | ||
+ | ==== Piezos ==== | ||
+ | |||
+ | The piezo objective movers are the most fragile and failure-prone component of the diSPIM. | ||
+ | |||
+ | ==== Software ==== | ||
+ | |||
+ | If you encounter bugs in the software it is best to contact the developers directly. | ||
+ | |||
+ | ==== Vibration ==== | ||
+ | |||
+ | Some users have reported vibrations, e.g. diffraction-size beads will appear as a diagonal smear. | ||
+ | |||
+ | There are three approaches to reduce the apparent vibration: | ||
+ | |||
+ | - **Reduce camera vibration: | ||
+ | - **Mechanically decouple the camera and piezo:** You can mount the camera to the air table instead of the the microscope. | ||
+ | - **Reduce susceptibility of piezo to vibrate:** You can update the piezo objective mover to the version introduced early 2015 which is significantly stiffer. |