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Try to isolate the problem to the extent possible.

Problem Possible Reason Possible Fix
Communication error between software and hardware Power cycle the affected hardware and restart the software
One hardware element not responding Connect to the hardware using manufacturer's software or serial terminal for Tiger controller
Beams only come out of the objective if both beams are turned on Fibers going to the wrong scanner Exchange the fibers
Stage scanning cameras not getting triggered Hardware/firmware out of date Make sure you have Rev F XY card or later, make sure micro-mirror card has jumper on positions 11/12 of SV6