Differences
This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
hardware:troubleshooting [2015/09/22 16:44] Jon Daniels |
hardware:troubleshooting [2016/02/16 20:02] Jon Daniels [Vibration] |
||
---|---|---|---|
Line 9: | Line 9: | ||
| Stage scanning cameras not getting triggered | Hardware/ | | Stage scanning cameras not getting triggered | Hardware/ | ||
| Acquisition images look different than alignment (look like epi view) | Camera trigger cables swapped | Swap the camera trigger cables | | | Acquisition images look different than alignment (look like epi view) | Camera trigger cables swapped | Swap the camera trigger cables | | ||
+ | | Inconsistent communication with Tiger controller | Windows comm port problems | Update USB to comm port [[http:// | ||
==== Piezos ==== | ==== Piezos ==== | ||
Line 18: | Line 19: | ||
If you encounter bugs in the software it is best to contact the developers directly. | If you encounter bugs in the software it is best to contact the developers directly. | ||
+ | ==== Vibration ==== | ||
+ | Some users have reported vibrations, e.g. diffraction-size beads will appear as a diagonal smear. | ||
+ | |||
+ | There are three approaches to reduce the apparent vibration: | ||
+ | |||
+ | - **Reduce camera vibration: | ||
+ | |||
+ | - **Mechanically decouple the camera and piezo:** You can mount the camera to the air table instead of the the microscope. | ||
+ | |||
+ | - **Reduce susceptibility of piezo to vibrate:** You can update the piezo objective mover to the version introduced early 2015 which is significantly stiffer. |