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Table of Contents
Troubleshooting
Try to isolate the problem to the extent possible.
| Problem | Possible Reason | Possible Fix |
|---|---|---|
| Communication error between software and hardware | Power cycle the affected hardware and restart the software | |
| One hardware element not responding | Connect to the hardware using manufacturer's software or serial terminal for Tiger controller | |
| Beams only come out of the objective if both beams are turned on | Fibers going to the wrong scanner | Exchange the fibers |
| Stage scanning cameras not getting triggered | Hardware/firmware out of date | Make sure you have Rev F XY card or later, make sure micro-mirror card has jumper on positions 11/12 of SV6 |
| Acquisition images look different than alignment (look like epi view) | Camera trigger cables swapped | Swap the camera trigger cables |
Piezos
The piezo objective movers are the most fragile and failure-prone component of the diSPIM. See the wiki page for care and troubleshooting instructions.
Software
If you encounter bugs in the software it is best to contact the developers directly. For Micro-Manager see the wiki page for instructions.
