This is an old revision of the document!
Objectives
The choice of light sheet objectives is limited because they must be co-focused without bumping into each other. A detailed overview is given in Supplementary Note 6 in the Power/Huisken review paper (link to supplemental).
The most commonly-used objectives for (symmetric) diSPIM are 40x water-dipping objectives with a NA of 0.8 (Nikon CFI Apo 40XW NIR). The Olympus 20x/0.5 water objective is another possibility) as is the Nikon 10x/0.3 water. ASI and Special Optics have co-developed an objective for cleared tissue that is suitable for the diSPIM geometry and can image cleared tissue up to 5 mm deep in slab form or within a 12 mm spherical envelope.
Single-sided systems (iSPIM) have much more flexibility because the illumination objective can be a low-NA long-WD objective. A popular pair for high-resolution imaging is the same objective pair as used on the Lattice light sheet, specifically the Nikon 25x/1.1 objective paired with Special Optics 54-10-7 which is 28.6x/0.66.
